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Description:
Recent advances in long-read sequencing technologies allows for a more complete identification of DNA and RNA associated with human disease. Two talks will be given to describe a few applications of these technologies.
Dr. Brooks will describe nanopore sequencing of full-length cDNA from chronic lymphocytic leukemia (CLL) with and without mutation in a splicing factor, SF3B1. SF3B1 is one of the most frequently mutated genes in and is associated with poor patient prognosis. While alternative splicing patterns caused by mutations in SF3B1 using short-read sequencing are known, identifying the full-length isoform changes may better elucidate the functional consequences of these mutations. The talk will describe a computational workflow for transcript analysis called FLAIR (Full-Length Alternative Isoform analysis of RNA), novel findings, and remaining challenges with long-read sequencing approaches for alternative splicing analysis.
Dr. Bloom will talk about single-molecule real time sequencing of influenza. Infection by influenza virus can activate innate immune pathways that help control viral infection. However, these pathways are only rarely activated in single virus-infected cells. This webcast will describe how combining long-read sequencing with other single-cell techniques to determine how mutations in influenza virions affect whether infected cells activate innate immune pathways.
Learning Objectives:
- Describe long-read sequencing technology for both bulk and single-cell applications
- Describe application of long-read technology for cancer transcriptomics and to understand influenza infection
- Describe benefits and challenges of long-read sequencing
Moderator: Cecilia S. Yeung, MD
Duration: 1 hr
Level of Instruction: Basic
Date Recorded: August 6, 2019
Planned and coordinated by the Training and Education Committee
Continuing Education Credit Information
CE Credit for this course has expired.Note: Members of AMP can access this webinar at no cost. Join the AMP Family!
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