Please note that this content was created in 2018. In the time since this material was posted, there may have been additional developments, advancements, and/or more current publications in this field.
AMP Education is constantly updating our educational offerings, and will remove or replace content that is no longer accurate. Please be sure to use the search function to find related or updated material available in our catalogue at educate.amp.org. The Training and Education Committee works with AMP Education Programs to bring you the most up-to-date and cutting-edge information on molecular pathology research, applications, and training.
This is a recording of a session from the AMP 2018 Annual Meeting & Expo. Purchase the entire AMP Annual Meeting & Expo 2018 Recordings for a significant discount!
A number of DNA viruses, including Hepatitis B virus (HBV) and Human papillomavirus (HPV), cause severe, chronic diseases in humans that are difficult to cure using currently available approaches. One possible novel treatment approach involves the cleavage and destruction of the long-lived viral DNA genomes that maintain these diseases using DNA editing. This session will discuss data obtained in cultured cells and animals that demonstrate significant reductions in viral load after targeting of the HBV or HPV16 DNA genome using CRISPR/Cas. The aim of this session is to also present studies performed to understand the molecular mechanisms by which CRISPR-Cas proteins such as Cas9 and Cas13 are able to target RNA, and how these properties can be exploited to develop a number of applications including RNA detection, RNA imaging, and manipulation of RNA function in health and disease.
CRISPR/Cas9 Targeting and Inactivation of Viral DNA Genomes
Bryan R. Cullen, PhD, Duke University Medical Center, Durham, NC, USA
Programmable RNA-targeting CRISPR-Cas Enzymes for RNA Detection and Therapeutics
Mitchell R. O'Connell, PhD, University of Rochester, Rochester, NY, USA
Objectives:
- Summarize the molecular basis for persistent infections caused by DNA viruses.
- Outline evidence that CRISPR/Cas represents a potentially useful approach to the treatment and possibly even cure of otherwise refractory DNA virus infections.
- Discuss the molecular mechanisms of specific interaction between CRISPR/Cas9 and Cas13 adaptive immune systems and RNA.
- Outline the use of these properties to develop a number of applications including RNA detection, RNA imaging and manipulation of RNA function in health and disease.
Duration: 1.25 hr
Recording Date: November 2, 2018
Last day to purchase: December 31, 2021
There are no CME/CMLE or SAMs credits available.Note: Join the AMP Family for discounted access to the most current educational resources!
All sales are final. No refunds will be issued.
No digital files may be reproduced or transmitted in any form, by any means, electronic or mechanical. By purchasing a product, you agree to not share any of the course materials, including videos, downloadable slide presentations, outlines, manuscripts, etc. without explicit and written permission from AMP.
