Making the most out of the least has long been a requirement for the practical application of molecular biology. The technology arc of my career from recombinant DNA to Ancient DNA to PCR to forensic DNA to pathogen detection to Next-Generation Sequencing – has been anchored in this consistent need to deal with samples with limited nucleic acid content. In describing this arc, I will present a personal journey that shows, with respect to getting the most information from our samples, how far we’ve come over the course of my career. I will also describe the early, heady days of PCR and the invention of real-time PCR, the application of real-time PCR to real-world problems (including those of the developing world) and my recent work on making PCR faster on existing instruments.
Efficient Use of the Available DNA - A Career
Russell Higuchi, PhD, Cepheid, Sunnyvale, CA, USA
Objectives:
- Describe the history of sensitive DNA detection and sequence identification.
- Provide a review of the principles of real-time PCR detection and quantification.
- Look forward to better, faster and cheaper molecular diagnostic tools.
Duration: 1.00 hr
Recording Date: November 7, 2019
CME/CMLE credit: 1.00 hr
Last day to purchase course and CE claim credit: December 24, 2022
